Rupp was born September 2, 1901 in Halstead, Kansas to Heinrich Rupp, a German immigrant, and Anna Lichi, a Palatinate (Quirnheim, Germany) immigrant. The fourth of six children, Rupp grew up on a 163-acre farm that his parents had homesteaded. He began playing basketball as a young child, with the help of his mother, who made a ball for him by stuffing rags into a gunnysack. "Mother sewed it up and somehow made it round," he recalled in 1977. "You couldn't dribble it. You couldn't bounce it either."
Rupp died of spinal cancer at age 76 in Lexington, Kentucky, on December 10, 1977, on a night when Kentucky defeated his alma mater, Kansas, at Allen Fieldhouse in Lawrence, Kansas. The game that night was promoted as "Adolph Rupp Night". He is buried in Lexington Cemetery. Rupp Arena, the current home of the Kentucky men's basketball team, is named in his honor.