Macqueen has appeared in television advertisements for Specsavers, Dell, and Utterly Butterly. He appeared in the Comedy Central/BBC collaboration, Kröd Mändoon and the Flaming Sword of Fire, in which he played Barnabus, the sidekick to the Chancellor Dongalor, played by Matt Lucas. He starred as Roy Tunt in The Hide, which premiered on FilmFour in February 2009. He won the award for Best Actor at the Marbella Film Festival in 2009 for this role.
Macqueen has appeared in a series of videos on YouTube as Clive Rudloe, a character who claims to be "the world's number one DJ". The videos are spoofs created by real-life DJs Above and Beyond. In late 2009, he starred in several episodes of The News at Bedtime on BBC Radio 4, in a variety of supporting roles.