Farkash born in Toronto, Ontario, to a family of Jewish descent. At the age of two years, she was living with her family in Ramat Hasharon, Caesarea, Israel. At thirteen, she began to study singing. She became known in 2006 when she recorded the song Millions of Stars in memory of her brother, Captain Tom Farkash, who died in a helicopter crash during the Second Lebanon War in 2006. On hearing of his death, Amit asked a friend of Tom to write the song, being sung by Amit for the first time at his funeral. It gained fame on radio and became the song most closely identified with the war.

From 2007 to 2008 Farkash played a lead role in the Israeli version of High School Musical. She also performed at the Hanukkah Festigal in 2009 and 2011. In 2010 she published her debut album Feel and released the single Something New.

In 2009 Farkash got her first big lead role in TV. She co-starred with Yon Tumarkin in the Israeli drama series Split. The series has been a huge international success and was sold to 78 different countries. In 2012 Amit Farkash was a contestant in season seven of the Israeli series Dancing With the Stars. She was partnered with professional dancer Oron Dahan and won the third place. Since 2014 Amit is portraying Dana in the TV series The Nerd Club.