Jackson began to show his true potential in 1989, when he was voted to start for the American League All-Star team, and was named the game's MVP for his play on both offense and defense. In the top of the first inning, he caught Pedro Guerrero's 2-out line drive to left-center field to save two runs. Then he led off the bottom of the first—his first All-Star plate appearance—with a monstrous 448-foot (137 m) home run off Rick Reuschel of the San Francisco Giants. NBC-TV announcer Vin Scully exclaimed, "Look at that one! Bo Jackson says hello!" Wade Boggs followed with his own home run, making them the first pair in All-Star history to lead off their side's first with back-to-back home runs. In the 2nd inning, he beat out the throw on a potential double play to drive in the eventual winning run. He then stole second base, making him the second player in All-Star Game history to hit a home run and steal a base in the same game (the first was Willie Mays). Jackson finished the game with two hits in four at-bats, one run scored, and two RBI.
Jackson's 171 strikeouts in 1989 tied him for 10th most strikeouts in a season for a right-handed batter since 1893. On July 11, 1990, against the Orioles, Jackson performed his famous "wall run", when he caught a ball six strides away from the wall. As he caught the ball at full tilt, Jackson looked up and ran three steps along the wall, to avoid impact and the risk of injury from the fence.
In his eight baseball seasons, Jackson had a career batting average of .250, hit 141 home runs and had 415 RBIs, with a slugging average of .474. His best year was 1989, with his effort earning him All-Star status. In 1989, Jackson ranked fourth in the American League in both home runs, with 32, and RBI, with 105.
Jackson's 1989 season was his best in the league. In eleven games, with nine starts, Jackson rushed for a total of 950 yards with a 5.5 yards per carry average and four touchdowns. In his abbreviated 1990 campaign, Jackson rushed for 698 yards and was selected to the only Pro Bowl of his career.
Called "the greatest athlete in video game history", Jackson's digital counterpart was nicknamed by fans as "Tecmo Bo" since being featured in the 1989 video game Tecmo Bowl for the Nintendo Entertainment System, where he was all but untacklable. Players would make the popular move of running Bo all the way back to his own 1 yard line, then run 99 yards for a touchdown with defenders literally bouncing off him. Referencing his video game character, Jackson was featured in a 2016 advertisement for the Kia Sorento, with Jackson driving the car into a virtual stadium (a second ad features Brian Bosworth with Jackson referencing the infamous Monday Night Football touchdown run).