Morukov graduated from high school in 1967 and received his M.D. from the 2nd Moscow Medical Institute (now Russian State Medical University) in 1973. He joined the professorate in space, aviation and naval medicine at the Institute for Biomedical Problems in 1978 and received a Ph.D in these disciplines in 1979. As a cosmonaut-physician, Morukov completed medical training in cardiology, gastroenterology, otolaryngology, stomatology, ophthalmology, and cardiopulmonary resuscitation between 1989–91. In 1995, he took an advanced course in emergency medical care. In 1996, he completed a medical training course in endocrinology and hematology.