Born in Buffalo, New York as Robert Emil Schmidt, he attended Masten Park High School. Schmidt got his start in radio in Buffalo. He started at WGR (AM) but switched from WGR to WBEN’s late morning radio slot in 1943, as part of a move which also brought Clint Buehlman’s early morning show over from WGR to WBEN at the same time. (The WBEN morning slot had opened when its host, future NBC-TV personality Jack Paar, was drafted into the military.)

The puppet Howdy Doody was based on a caricature of Mr. Smith’s sister, Esther. She was employed at a department store and Howdy was the spitting image of her. Smith also was known as a singer and musician, appearing on many top shows of the time before and even after becoming nationally known for the Howdy Doody show. At first it aired on Saturdays, then on Mondays, Wednesdays and Fridays, and finally, five times a week. In 1954, Smith suffered a heart attack and for a time, he did the show from a studio built in the basement of his home in Mount Vernon, New York. He returned to the NBC studio in 1955. The final NBC Howdy Doody episode aired in 1960. Later, in 1976, Smith reunited with longtime show producer Roger Muir and several of the original cast to produce a new daily syndicated Howdy Doody show.

In 1970 and 1971, Smith embarked on a live tour of college campuses. The shows, organized by producer Burt DuBrow, mixed nostalgia with more contemporary humor, such as Buffalo Bob finding a package of Zig Zags (rolling paper) allegedly belonging to Clarabell. One show, on April 4, 1971, was recorded and released as an LP, on the Project 3 Total Sound Stereo label. It was titled Buffalo Bob Smith Live at Bill Graham’s Fillmore East.

He made a live infomercial appearance to promote Howdy Doody Entertainment Memorabilia on July 3, 1998, on QVC. That was his last appearance. Smith died of cancer a few weeks later on July 30, 1998, in a hospital in Hendersonville, North Carolina, just three days before puppeteer Shari Lewis, whose show took over the time slot that Howdy Doody had previously occupied.