Ridenhour was born in Queens, New York. He began writing rhymes after the New York City blackout of 1977. After attending W. Tresper Clarke High School, he went to Adelphi University on Long Island to study graphic design, where he met William Drayton (Flavor Flav). He received a B.F.A. from Adelphi in 1984 and later received an honorary doctorate from Adelphi in 2013.

Ridenhour (using the nickname Chuck D) formed Public Enemy in 1985 with Flavor Flav. Upon hearing Ridenhour’s demo track “Public Enemy Number One”, fledgling producer/upcoming music-mogul Rick Rubin insisted on signing him to his Def Jam label. Their major label releases were Yo! Bum Rush the Show (1987), It Takes a Nation of Millions to Hold Us Back (1988), Fear of a Black Planet (1990), Apocalypse 91… The Enemy Strikes Black (1991), the compilation album Greatest Misses (1992), and Muse Sick-n-Hour Mess Age (1994). They also released a full-length album soundtrack for the film He Got Game in 1998.

Ridenhour also contributed (as Chuck D) to several episodes of the PBS documentary series The Blues. He has appeared as a featured artist on many other songs and albums, having collaborated with artists such as Janet Jackson, Kool Moe Dee, The Dope Poet Society, Run–D.M.C., Ice Cube, Boom Boom Satellites, Rage Against the Machine, Anthrax, John Mellencamp and many others. In 1990, he appeared on “Kool Thing”, a song by the alternative rock band Sonic Youth, and along with Flavor Flav, he sang on George Clinton’s song “Tweakin'”, which appears on his 1989 album The Cinderella Theory. In 1993, he executive produced Got ‘Em Running Scared, an album by Ichiban Records group Chief Groovy Loo and the Chosen Tribe.

Chuck D identifies as Black, as opposed to African or African-American. In a 1993 issue of DIRT Magazine covering a taping of In the Mix hosted by Alimi Ballard at the Apollo, Dan Field writes,

In 1996, Ridenhour released Autobiography of Mistachuck on Mercury Records. Chuck D made a rare appearance at the 1998 MTV Video Music Awards, presenting the Video Vanguard Award to the Beastie Boys, whilst commending their musicianship. In November 1998, he settled out of court with Christopher “The Notorious B.I.G.” Wallace’s estate over the latter’s sampling of his voice in the song “Ten Crack Commandments”. The specific sampling is Ridenhour counting off the numbers one to nine on the track “Shut ‘Em Down”. He later described the decision to sue as “stupid”.

In September 1999, he launched a multi-format “supersite” on the web site Rapstation.com. The site includes a TV and radio station with original programming, prominent hip hop DJs, celebrity interviews, free MP3 downloads (the first was contributed by multi-platinum rapper Coolio), downloadable ringtones by ToneThis, social commentary, current events, and regular features on turning rap careers into a viable living. Since 2000, he has been one of the most vocal supporters of peer-to-peer file sharing in the music industry.

Addressing the negative views associated with rap music, he co-wrote the essay book Fight the Power: Rap, Race, and Reality with Yusuf Jah. He argues that “music and art and culture is escapism, and escapism sometimes is healthy for people to get away from reality”, but sometimes the distinction is blurred and that’s when “things could lead a young mind in a direction.” He also founded the record company Slam Jamz and acted as narrator in Kareem Adouard’s short film Bling: Consequences and Repercussions, which examines the role of conflict diamonds in bling fashion. Despite Chuck D and Public Enemy’s success, Chuck D claims that popularity or public approval was never a driving motivation behind their work. He is admittedly skeptical of celebrity status, revealing in a 1999 interview with BOMB Magazine that, “The key for the record companies is to just keep making more and more stars, and make the ones who actually challenge our way of life irrelevant. The creation of celebrity has clouded the minds of most people in America, Europe and Asia. It gets people off the path they need to be on as individuals.”

He loaned his voice to Grand Theft Auto: San Andreas as DJ Forth Right MC for the radio station Playback FM. In 2000, he collaborated with Public Enemy’s Gary G-Whiz and MC Lyte on the theme music to the television show Dark Angel. He appeared with Henry Rollins in a cover of Black Flag’s “Rise Above” for the album Rise Above: 24 Black Flag Songs to Benefit the West Memphis Three. In 2003, he was featured in the PBS documentary Godfathers and Sons in which he recorded a version of Muddy Waters’ song “Mannish Boy” with Common, Electrik Mud Cats, and Kyle Jason. He was also featured on Z-Trip’s album Shifting Gears on a track called “Shock and Awe”; a 12-inch of the track was released featuring artwork by Shepard Fairey. In 2008 he contributed a chapter to Sound Unbound: Sampling Digital Music and Culture (The MIT Press, 2008) edited by Paul D. Miller a.k.a. DJ Spooky, and also turned up on The Go! Team’s album Proof of Youth on the track “Flashlight Fight.” He also fulfilled his childhood dreams of being a sports announcer by performing the play-by-play commentary in the video game NBA Ballers: Chosen One on Xbox 360 and PlayStation 3.

Ridenhour is politically active; he co-hosted Unfiltered on Air America Radio, testified before Congress in support of peer-to-peer MP3 sharing, and was involved in a 2004 rap political convention. He has continued to be an activist, publisher, lecturer, and producer.

In an interview with the magazine N’Digo published in June 2008, he spoke of today’s mainstream urban music seemingly relishing the addictive euphoria of materialism and sexism, perhaps being the primary cause of many people harboring resentment towards the genre and its future. However, he has expressed hope for its resurrection, saying “It’s only going to be dead if it doesn’t talk about the messages of life as much as the messages of death and non-movement”, citing artists such as NYOil, M.I.A. and The Roots as socially conscious artists who push the envelope creatively. “A lot of cats are out there doing it, on the Web and all over. They’re just not placing their career in the hands of some major corporation.”

In 2009, Ridenhour wrote the foreword to the book The Love Ethic: The Reason Why You Can’t Find and Keep Beautiful Black Love by Kamau and Akilah Butler. He also appeared on Brother Ali’s album, Us.

In 2010, Chuck D released a track, “Tear Down That Wall.” He said, “I talked about the wall not only just dividing the U.S. and Mexico but the states of California, New Mexico and Texas. But Arizona, it’s like, come on. Now they’re going to enforce a law that talks about basically racial profiling.”

In March 2011, Chuck D re-recorded vocals with The Dillinger Escape Plan for a cover of “Fight the Power”.

Chuck D duetted with Rock singer Meat Loaf on his 2011 album Hell in a Handbasket on the song “Mad Mad World/The Good God Is a Woman and She Don’t Like Ugly”.

In 2016 Chuck D joined the band Prophets of Rage along with B-Real and former members of Rage Against the Machine.

In July 2019, Ridenhour sued Terrordome Music Publishing and Reach Music Publishing for $1 million for withholding royalties.