In 2016, she scored four goals, tied for second most on the team. Matheson currently holds the records for most goals scored (23) and most assists (12) for the Spirit all-time. In 2016 the Washington Spirit advanced to the NWSL Championship game against the Western New York Flash. After extra time the game was tied 2–2 and advanced to penalties. Matheson was the fifth penalty taker for the Spirit, Washington was trailing 2–3 and needed to score to keep the match going. Her penalty was saved by Sabrina D'Angelo, securing the win for the Flash.
Matheson was named to her third Olympic team in 2016, where Canada won the bronze medal for the second consecutive Olympics. Matheson earned her 200th cap for Canada on October 14, 2018, 181 of those caps were starts.