Harron’s parents owned and operated Harron’s Cleaners and Dryers in Toronto. Beginning at the age of ten, he earned extra money for the family during the Great Depression, doing “chalk talks” telling humorous stories while drawing caricatures in coloured chalk at company or club banquets, making $10 or $15 a talk. As a result of his performances, he was invited to audition for, and won, a part in the Canadian Radio Broadcasting Commission radio series Lonesome Trail in 1935.

As a teenager, Harron spent time working as a farm hand in rural Ontario; experience he later credited for the development of his Charlie Farquharson character. He graduated from Vaughan Road Collegiate Institute in 1942 and briefly attended the University of Toronto before enlisting in the Royal Canadian Air Force in 1943. After the Second World War, he completed his studies of sociology and philosophy receiving a Bachelor of Arts degree. While at school he performed in amateur and professional productions, won the Victoria College drama award, and composed the music and lyrics for a student musical. He won the gold medal in philosophy and the regent’s silver medal and was offered a position teaching literature at the university which he turned down in order to focus on performing.

Harron is known for the character Charlie Farquharson /ˈfɑːrkəsən/, a personality he first portrayed in 1952 on the CBC series The Big Revue. For the following half-century-plus, Harron performed the character regularly on stage and on Canadian radio and television. As well, the character received international attention as part of the cast of the U.S. country music television show, Hee Haw during its 23-year run; on that series, which ran from 1969 to 1992, Harron portrayed a rural anchorman for station KORN, and concluded the final story of each newscast with a cutthroat gesture. Harron reprised the character on The Red Green Show in 2003 and 2004.

Returning to North America in the 1950s, Harron was featured in the inaugural season of the Stratford Shakespeare Festival in Ontario as the male lead in All’s Well That Ends Well and a minor part in Richard III and on Broadway and was one of the writers on the first English-language dramatic series broadcast in Canada, Sunshine Sketches, which aired from 1952 to 1953 on CBC Television. Harron also co-wrote the script for the 1956 television musical Anne of Green Gables. Harron later adapted the production for the stage in 1965 as Anne of Green Gables: The Musical, which continues to be performed annually during the Charlottetown Festival. According to Harron in a 2008 interview with the Calgary Herald, the stage show has provided work for more than 10,000 actors since its inception.

Harron’s first marriage was to Gloria Fisher, his second from 1960-1968 was to actress Virginia Leith. He married his third wife, Canadian singer Catherine McKinnon in 1969. Harron and McKinnon divorced in 2003. He moved in with and later married his fourth wife, Claudette Gareau, who had played the separatist weather girl in Shh! It’s the News (1973) appearing with Harron.

He made one appearance on the CBC Television show Adventures in Rainbow Country in the episode “The Frank Williams File” (1969). He has also been a host and interviewer on Canadian television and radio, hosting CBC Radio’s Morningside from 1977 to 1982, for which he received an ACTRA Award for best radio host, and subsequently hosting an afternoon talk show, The Don Harron Show on CTV from 1983 to 1985. He had a featured role in Arthur Hiller’s film The Hospital (1971), written by Paddy Chayefsky. He replaced Gene Wood as host of the game show Anything You Can Do from 1972 to 1974.

In 2000, Harron’s contribution to the Canadian entertainment industry was recognized with his being named a member of the Order of Ontario. He was invested as member of the Order of Canada in 1980 and in 2007, he was given the Gemini Award for Lifetime Achievement in Radio and Television. Harron was also appointed a UNICEF Goodwill Ambassador. In his later years, he was a high-profile advocate for the interests of older people. He also continued to write books, most recently (2008) publishing a retrospective work on the history of the Anne of Green Gables musical to tie in with the 100th anniversary of the original novel.

Harron was inducted into the Canadian Country Music Hall of Fame in 2010.

On May 15, 2011, Don performed the Charlie Farquharson character during the 85th anniversary of his Toronto high school, Vaughan Road Collegiate Institute. His commentary included a reference to former US president Bill Clinton’s sexual dalliance with Monica Lewinsky. “He deified yer 11th Commandment – Thou shalt not put thy rod before thy staff.”