Eusébio was born in the Mafalala neighbourhood, Lourenço Marques (now Maputo), Portuguese Mozambique on 25 January 1942. His parents were Laurindo António da Silva Ferreira, a white railroad worker from Malanje, Angola, and Elisa Anissabeni, a black Mozambican woman. He was Elisa’s fourth child. Raised in an extremely poor society, he used to skip school classes to play barefoot football with his friends on improvised pitches and using improvised footballs. His father died from tetanus when Eusébio was eight years old, so Elisa almost exclusively took the parental care of young Eusébio.

Eusébio played for two seasons with their youth team, while he made sporadic appearances in the senior team. There he won the Campeonato Provincial de Moçambique and the Campeonato Distrital de Lourenço Marques in his last season, in 1960.

Eusébio moved to Lisbon in his late teens, after joining Benfica as an 18-year-old from his local club, Sporting de Lourenço Marques, for 350,000 Portuguese escudos (equivalent to €136,000 in 2009). Benfica discovered Eusébio due to the efforts of Brazilian former player José Carlos Bauer, who saw him in Lourenço Marques in 1960. Eusébio could run 100 metres in under 11 seconds. Although he preferred playing with the right side foot, Eusébio could use the left side just as well. At times, Eusébio would surprise opponents with his dribbling ability, seemingly, a talent he preferred to keep secret. Bauer recommended Eusébio first to his former club, São Paulo, but the Tricolor turned him down.

Benfica only registered him in May the next year, and Eusébio made his first appearance for them against Atlético Clube de Portugal in a friendly game on 23 May 1961. He scored a hat-trick in a 4–2 victory. His debut in an official match was on 1 June 1961, against Vitória de Setúbal, in the third round second leg of the 1960–61 Taça de Portugal. The game was controversially scheduled for the day after the European Cup final against Barcelona and the Portuguese Football Federation did not postpone it. As the first team was returning from Bern, Benfica played with the reserve squad and was defeated 1–4. Eusébio scored a goal and missed a penalty (the first of only five he missed throughout his career), but this was not enough to win the round (4–5 on aggregate). On 10 June 1961, Eusébio played for the first time in the Primeira Divisão, the last match day against Belenenses, where he scored a goal in a 4–0 win. On 15 June, Benfica played the final of the invitational Tournoi de Paris against Pelé’s Santos, and in the beginning of the second half, with Benfica down 0–4, Béla Guttmann decided to bring Eusébio from the bench to substitute Santana. Shortly after coming in, Santos reached 0–5. However, between the 63rd and the 80th minute, Eusébio scored 3 goals and suffered a foul inside the penalty area, the penalty taker, José Augusto, failed to score though. The game finished 6–3 for Santos, with Eusébio being on the cover of the famed French sporting newspaper L’Équipe.

His following season was the one where he started to gain global recognition among football fans and critics alike. He scored 12 goals in 17 league matches, and even though the club finished third, they won the Taça de Portugal against Vitória de Setúbal, with Eusébio scoring two goals in the final. In that same season, he won the European Cup, also scoring two goals in the final against Real Madrid in a 5–3 result to Benfica. Due to his fine form during the season, he finished second in the 1962 Ballon d’Or, in his first complete season as a professional. In October 1963, he was selected to represent the FIFA team in the “Golden Anniversary” of The Football Association at Wembley Stadium.

He received a number of individual accolades and awards while playing for Benfica. He was the 1965 European Footballer of the Year (Ballon d’Or) and finished as runner-up twice, in 1962 and 1966, and in 1968 was the first winner of the Golden Boot Award, as Europe’s leading scorer, a feat he repeated five years later. He was the Portuguese First Division’s top scorer seven times (1964, 1965, 1966, 1967, 1968, 1970 and 1973), helping Benfica to win 11 Primeira Liga (1960–61, 1962–63, 1963–64, 1964–65, 1966–67, 1967–68, 1968–69, 1970–71, 1971–72, 1972–73 and 1974–75), 5 Portuguese Cup wins (1961–62, 1963–64, 1968–69, 1969–70 and 1971–72), 1 European Cup win (1961–62) and 3 European Cup finals (1962–63, 1964–65 and 1967–68).

Benfica were also European Cup runners-up in 1963, 1965 and 1968. In the 1968 defeat to the English league champions Manchester United at Wembley Stadium, with the scores 1–1, he came close to winning the game for Benfica in the dying seconds of the game, only to have his shot saved by Alex Stepney. Despite this, and the fact that the English side went on to win 4–1 in extra time, he openly congratulated Stepney for his efforts throughout the game, stopping to applaud Stepney, as he threw the ball back into play.

Eusébio was a devout Roman Catholic and married Flora Claudina Burheim in 1965.

He also played in the North American Soccer League (NASL), for three different teams, from 1975 to 1977: Boston Minutemen (1975), Toronto Metros-Croatia (1976), and the Las Vegas Quicksilvers (1977). His most successful season in the NASL was in 1976 with Toronto Metros-Croatia. He scored the winning goal in their 3–0 victory at the Soccer Bowl ’76 to win the NASL title. The same year, he played ten games for Monterrey in the Mexican league.

Although his knees robbed him of his ability to continue in the NASL, Eusébio wanted to continue to play soccer. He found a home in 1978 with the New Jersey Americans of the second-tier American Soccer League (ASL). He went on to play five games for the Buffalo Stallions during the 1979–80 Major Indoor Soccer League season. He retired in 1979 and formed part of the technical committee of the Portugal national football team.

In addition to winning the Golden Boot (with nine goals) for the 1966 World Cup, Eusébio also set a record that year for the most penalties scored (shoot-out not included), with four. Eusébio’s four goals against North Korea in the quarter-final match also helped Portugal tie the record for largest deficit overcome in a win (three goals, equaling Austria in 1954) and he became the fifth player to score as many goals in a FIFA World Cup match, a record he jointly held until Oleg Salenko scored five in the 1994 World Cup. The English were so impressed by Eusébio’s performances that his waxwork was immediately added to the Madame Tussauds in London. He also received the BBC Overseas Sports Personality of the Year for 1966.

Eusébio was the leading scorer for his country, with 41 goals in 64 matches, until Pauleta equalled and surpassed his record against Latvia on 12 October 2005. Eusébio was also the most capped Portuguese player from 1972, until Tamagnini Nené made his 64th cap against Yugoslavia on 2 June 1984 in a friendly match, breaking Eusébio’s record during the UEFA Euro 1984 on 20 June against Romania. He made his debut for the Portugal national team against Luxembourg on 8 October 1961, a match his country lost 4–2, with the player scoring his country’s first goal in the match.

Eusébio died at his home on 5 January 2014 of heart failure, aged 71. Many well-known people from the football world expressed their condolences and praise, including his contemporaries Franz Beckenbauer and Bobby Charlton. The Portuguese Football Federation made a statement and said that a moment of silence was to be observed in Portuguese football matches. There were tributes from Cristiano Ronaldo, Luís Figo, José Mourinho, Carlos Queiroz, FIFA President Sepp Blatter UEFA president Michel Platini, Greg Dyke, and former President of Mozambique and childhood friend Joaquim Chissano.

Following Eusébio’s death, the Portuguese government declared three days of national mourning. Hundreds of thousands paid tribute to him. On 6 January 2014, a mass was held at the Igreja do Seminário do Largo da Luz. On 9 January, one of his wishes was granted as his coffin was transported around the field of the Estádio da Luz. Three days later, his statue (inaugurated on his 50th birthday) at the Praça Centenarium was transformed into a memorial. Hours later, the name “Eusébio” was on the back of Benfica players’ shirts during “O Clássico”.

In September 2019, Eusébio was, along with Maria Mutola, referred by Pope Francis as an example of perseverance, during the latter’s visit to Mozambique.