O'Keeffe, most famous for her depiction of flowers, made about 200 flower paintings, which by the mid-1920s were large-scale depictions of flowers, as if seen through a magnifying lens, such as Oriental Poppies and several Red Canna paintings. She painted her first large-scale flower painting, Petunia, No. 2, in 1924 that was first exhibited in 1925. Making magnified depictions of objects created a sense of awe and emotional intensity. On November 20, 2014, O'Keeffe's Jimson Weed/White Flower No 1 (1932) sold for $44,405,000 in 2014 at auction to Walmart heiress Alice Walton, more than three times the previous world auction record for any female artist.
In 1924, Stieglitz arranged a simultaneous exhibit of O'Keeffe's works of art and his photographs at Anderson Galleries and arranged for other major exhibits. The Brooklyn Museum held a retrospective of her work in 1927. In 1928, he announced to the press that six of her calla lily paintings sold to an anonymous buyer in France for US$25,000, but there is no evidence that this transaction occurred the way Stieglitz reported. However, due to the press, O'Keeffe's paintings sold at a higher price from that point onward. By the late twenties she was noted for her work as an American artist, particularly for the paintings of New York city skyscrapers and close-up paintings of flowers.
In 1924, Stieglitz was divorced from his wife Emmeline, and he married O'Keeffe. For the rest of their lives together, their relationship was, "a collusion... a system of deals and trade-offs, tacitly agreed to and carried out, for the most part, without the exchange of a word. Preferring avoidance to confrontation on most issues, O'Keeffe was the principal agent of collusion in their union," according to biographer Benita Eisler. They primarily lived in New York City, but spent their summers at his family home, Oaklawn, in Lake George in upstate New York.