Presnell was born in 1933, in Modesto, California. He made his professional stage debut performing in an opera at the young age of sixteen. He attended the University of Southern California, earning a bachelor’s degree in vocal performance.

In 1956, Presnell co-starred with Herva Nelli in the American premiere of Darius Milhaud’s opera David at the Hollywood Bowl. In 1960, he was a featured soloist with the Los Angeles Philharmonic in another concert at the Bowl. In attendance at the concert was musical theater composer Meredith Willson who was at that time in the midst of creating his musical on the life of Molly Brown, Titanic survivor. Willson contacted him after the concert and asked him to audition for him. Presnell complied and Wilson ended up offering him the role of “Leadville Johnny.” At this point the musical was not completed and Wilson went on to compose much of the music for Leadville Johnny with Presnell’s voice in mind. On November 3, 1960, The Unsinkable Molly Brown opened on Broadway at the Winter Garden Theatre to a resounding success. Presnell stayed with the show for its entire 532 performance run, which ended on February 10, 1962. He reprised the role in the 1964 film The Unsinkable Molly Brown for which he won a Golden Globe Award for New Star of the Year.

He then pursued further vocal studies in Europe and at the Music Academy of the West. He appeared with a number of opera companies and orchestras during the 1950s. In 1957 he made his debut with the San Francisco Opera as the Officer in Richard Strauss’s Ariadne auf Naxos.

Presnell did some other film and television work in the 1960s and early 1970s, but for the next couple of decades concentrated primarily on stage work, playing Rhett Butler in the West End production of Scarlett and touring the United States as Daddy Warbucks in Annie and its sequel, Annie Warbucks, among other productions. In 1966, Presnell played the role of Sir Lancelot in Camelot in the Regional Equity production at The Houston Music Theatre.

He starred in two films in 1965: portraying Sol Rogers, a cavalry scout, in The Glory Guys and Danny Churchill, the romantic lead, in the musical film When the Boys Meet the Girls. He had one more sizable film role in the 1960s, the role of “Rotten Luck Willie” in Paramount’s 1969 musical Western film Paint Your Wagon, singing “They Call the Wind Maria”. The New York Times critic opined that Presnell’s role “delivered the golden opportunity to sing the unforgettable ballad.” Theater reviewer Thomas Hischak wrote “in one of the film’s few pleasing moments, Harve Presnell gave full voice to They Call the Wind Mariah and it was lovely to hear”. Referring to Eastwood and Marvin, film reviewer Brian W. Fairbanks wrote that “Harve Presnell steals both stars’ thunder with a knockout version of the best song”

In 1984, Presnell appeared as Don Quixote in Man of La Mancha in Darien, Connecticut, an appearance well received by The New York Times critic Alvin Klein, who wrote that Presnell was “a winning leading man”, and wrote:

Presnell died on June 30, 2009, aged 75, from pancreatic cancer at Saint John’s Health Center in Santa Monica, California.