Born in Macclesfield, Cheshire in 1933, Mayall was the son of Murray Mayall, a guitarist and jazz music enthusiast. From an early age, John was drawn to the sounds of American blues players such as Lead Belly, Albert Ammons, Pinetop Smith and Eddie Lang, and taught himself to play the piano, guitars, and harmonica.

In 1956, with college fellow Peter Ward, Mayall had founded the Powerhouse Four which consisted of both men and other local musicians with whom they played at local dances. In 1962 Mayall became a member of the Blues Syndicate. The band was formed by trumpeter John Rowlands and alto saxophonist Jack Massarik, who had seen the Alexis Korner band at a Manchester club and wanted to try a similar blend of jazz and blues. It also included rhythm guitarist Ray Cummings and drummer Hughie Flint, whom Mayall already knew. In 1962 John and his band were frequent and popular artists at all night R&B sessions at the ‘Twisted Wheel’ cellar club in central Manchester. Alexis Korner persuaded Mayall to opt for a full-time musical career and move to London, where Korner introduced him to many other musicians and helped them to find gigs. In late 1963, with his band, which was now called the Bluesbreakers, Mayall started playing at the Marquee Club. The line-up was Mayall, Ward, John McVie on bass and guitarist Bernie Watson, formerly of Cyril Davies and the R&B All-Stars. The next spring Mayall obtained his first recording date with producer Ian Samwell. The band, with Martin Hart at the drums, recorded two tracks : “Crawling Up a Hill” as well as “Mr. James”. Shortly after, Hughie Flint replaced Hart and Roger Dean took the guitar from Bernie Watson. This line-up backed John Lee Hooker on his British tour in 1964.

Mayall spent three years in Korea for national service and, during a period of leave, he bought his first electric guitar. Back in England, he enrolled at Manchester College of Art (now part of Manchester Metropolitan University) and started playing with semi-professional bands. After graduation, he obtained a job as an art designer but continued to play with local musicians. In 1963, he opted for a full-time musical career and moved to London. His previous craft would be put to good use in the designing of covers for many of his coming albums.

Mayall was offered a recording contract by Decca and, on 7 December 1964, a live performance of the band was recorded at the Klooks Kleek. A later studio-recorded single, “Crocodile Walk”, was released along with the album, but both failed to achieve any success and the contract was terminated.

In April 1965 former Yardbirds guitarist Eric Clapton replaced Roger Dean and John Mayall’s career entered a decisive phase.

In 1965, with Eric Clapton as their new guitar player, the Bluesbreakers began attracting considerable attention. That summer the band cut a couple of tracks for a single, “I’m Your Witchdoctor” b/w “Telephone Blues” (released in October). In August, however, Clapton left for a jaunt to Greece with a bunch of relative musical amateurs calling themselves the ‘Glands’. John Weider, John Slaughter, and Geoff Krivit attempted to fill in as Bluesbreaker guitarist but, finally, Peter Green took charge. John McVie was dismissed, and during the next few months Jack Bruce, from the Graham Bond Organisation, played bass.

In November 1965 Clapton returned, and Green departed as Mayall had guaranteed Clapton his spot back in the Bluesbreakers whenever he tired of the Glands. McVie was allowed back, and Bruce left to join Manfred Mann, but not before a live date by the Mayall-Clapton-Bruce-Flint line-up was recorded on Mayall’s two-track tape recorder at London’s Flamingo Club in November. The rough recording provided tracks that later appeared on the 1969 compilation Looking Back and the 1977 Primal Solos. The same line-up also entered the studio to record a planned single, “On Top of the World”, which was not released at that time. Mayall and Clapton cut a couple of tracks without the others (although some sources give this as occurring back in the summer): “Lonely Years” b/w “Bernard Jenkins” was released as a single the next August on producer Mike Vernon’s Purdah Records label (both tracks appeared again two decades later in Clapton’s Crossroads box set). In a November 1965 session, blues pianist-singer Champion Jack Dupree (originally from New Orleans but in the 1960s living in Europe) got Mayall and Clapton to play on a few tracks.

In April 1966 the Bluesbreakers returned to Decca Studios to record a second LP with producer Vernon. The sessions, with horn arrangements for some tracks (John Almond on baritone sax, Alan Skidmore on tenor sax, and Dennis Healey on trumpet), lasted just three days. Blues Breakers with Eric Clapton was released in the UK on 22 July 1966. Several of the 12 tracks were covers of pure Chicago blues (side 1 kicking off strong with Otis Rush’s “All Your Love” and Freddy King’s hit instrumental “Hide Away” [here spelled without a space as “Hideaway”]); Mayall wrote or arranged 5 (such as “Double Crossing Time”, a slow blues with a scorching solo by co-writer Clapton); and Eric debuted as lead vocalist, and began his practice of paying tribute to Robert Johnson, with “Ramblin’ on My Mind”. The album was Mayall’s commercial breakthrough, rising to No. 6 on the British chart, and has since gained classic status, largely for the audacious aggressiveness and molten fluidity of Clapton’s guitar playing. “It’s Eric Clapton who steals the limelight,” reports music mag Beat Instrumental, adding with unintended understatement, “and no doubt several copies of the album will be sold on the strength of his name.”

Mayall had to replace Clapton, and he succeeded in persuading Peter Green to come back. During the following year, with Green on guitar and various other sidemen, some 40 tracks were recorded. The album A Hard Road was released in February 1967. Today its expanded versions include most of this material, and the album itself also stands as a classic. In early 1967, Mayall released an EP recorded with American blues harpist Paul Butterfield.

In the meantime, on a single day in May 1967, Mayall had assembled a studio album to showcase his own abilities. Former Artwoods drummer Keef Hartley appeared on only half of the tracks, and everything else was played by Mayall. The album was released in November titled The Blues Alone.

A six-piece line-up—consisting of Mayall, Mick Taylor as lead guitarist, John McVie still on bass, Hughie Flint or Hartley on drums, and Rip Kant and Chris Mercer on saxophones—recorded the album Crusade on 11 and 12 July 1967. These Bluesbreakers spent most of the year touring abroad, and Mayall taped the shows on a portable recorder. At the end of the tour, he had over sixty hours of tapes, which he edited into an album in two volumes: Diary of a Band, Vols. 1 & 2, released in February 1968. Meanwhile, a few line-up changes had occurred: McVie had departed and was replaced by Paul Williams, who himself soon quit to join Alan Price and was replaced by Keith Tillman; Dick Heckstall-Smith had taken the sax spot.

Following a US tour, there were more line-up changes, starting with the troublesome bass position. First Mayall replaced bassist Tillman with 15-year-old Andy Fraser. Within six weeks, though, Fraser left to join Free and was replaced by Tony Reeves, previously a member of the New Jazz Orchestra. Hartley was required to leave, and he was replaced by New Jazz Orchestra drummer Jon Hiseman (who had also played with the Graham Bond Organisation). Henry Lowther, who played violin and cornet, joined in February 1968. Two months later the Bluesbreakers recorded Bare Wires, co-produced by Mayall and Mike Vernon, which came up to No. 6.

Hiseman, Reeves, and Heckstall-Smith then moved on to form Colosseum. The Mayall line-up retained Mick Taylor and added drummer Colin Allen (formerly of Zoot Money’s Big Roll Band / Dantalian’s Chariot, and Georgie Fame) and a young bassist named Stephen Thompson. In August 1968 the new quartet recorded Blues from Laurel Canyon.

On 13 June 1969, after nearly two years with Mayall, Taylor left and joined the Rolling Stones.

In November 1970 Mayall launched a recording project involving many of the most notable musicians with whom he had played during the previous several years. The double album Back to the Roots features Clapton, Mick Taylor, Gerry McGee and Harvey Mandel on guitar; Sugarcane Harris on violin; Almond on woodwinds; Thompson and Larry Taylor on bass; and Hartley on drums. Paul Lagos was with Sugarcane and ended up drumming on five. Mayall wrote all the songs and sang all the vocals, as usual by now, plus played harmonica, guitar, keyboards, drums, and percussion. The London sessions took place in January 1971 and as such represent some of Clapton’s last work before Derek and the Dominos’ attempted Layla follow-up sessions and band disintegration that spring.

By the start of the 1970s Mayall had relocated in the USA where he spent most of the next 15 years, recording with local musicians for various labels. In August 1971, Mayall produced a jazz-oriented session for bluesman Albert King and a few months later took on tour the musicians present in the studio.

A live album Jazz Blues Fusion was released in the following year, with Mayall on harmonica, guitar and piano, Blue Mitchell on trumpet, Clifford Solomon and Ernie Watts on saxophones, Larry Taylor on bass, Ron Selico on drums and Freddy Robinson on guitar. A few personnel changes are noted at the release of a similar album in 1973, the live Moving On. During the next decade Mayall continued shifting musicians and switching labels and released a score of albums. Tom Wilson, Don Nix and Allen Toussaint occasionally served as producers. At this stage of his career most of Mayall’s music was rather different from electric blues played by rock musicians, incorporating jazz, funk or pop elements and even adding female vocals. A notable exception is The Last of the British Blues (1978), a live album excused apparently by its title for the brief return to this type of music.

Since the end of the 1960s Mayall has lived in the US. A brush fire destroyed his house in Laurel Canyon in 1979, seriously damaging his musical collections and archives.

In 1982 Mayall was reunited with Mick Taylor, John McVie and Colin Allen, three musicians of his 1960s line-ups, for a two-year world tour from which a live album would emerge a decade later.

In 1984 Mayall restored the name Bluesbreakers for a line-up comprising the two lead guitars of Walter Trout and Coco Montoya, bassist Bobby Haynes and drummer Joe Yuele. The mythic name did, perhaps, something to enhance the interest in a band which by all standards was already remarkable.

Mayall tried a new format with lower volume, acoustic instruments, and no drummer. He recruited acoustic fingerstyle guitarist Jon Mark and flautist-saxophonist John Almond. Mark was best known as Marianne Faithfull’s accompanist for three years and for having been a member of the band Sweet Thursday (which included pianist Nicky Hopkins and future Cat Stevens collaborator Alun Davies, also a guitarist). Almond had played with Zoot Money and Alan Price and was no stranger to Mayall’s music—he had played baritone sax on 4 cuts of Blues Breakers with Eric Clapton and some of A Hard Road. This new band was markedly different from previous Mayall projects, and its making is well documented both on the 1999 double CD The Masters and on the 2004 DVD The Godfather of British Blues/The Turning Point.

On the occasion of the 40th year of his career, Mayall invited fellow musicians for the recording of a celebratory album. Along for the Ride appeared in 2001, credited to John Mayall and Friends with twenty names listed on the cover, including some Bluesbreakers, old and new, and also Gary Moore, Jonny Lang, Steve Cropper, Steve Miller, Otis Rush, Billy Gibbons, Greg Rzab, Chris Rea, Jeff Healey and Shannon Curfman.

To celebrate his 70th birthday, Mayall reunited with special guests Eric Clapton, Mick Taylor and Chris Barber during a fundraiser show. This “Unite for Unicef” concert took place on 19 July 2003 at the Liverpool Arena, and was captured on film for a DVD release.

In 2005 Mayall was appointed Officer of the Order of the British Empire (OBE) in the Honours List.

In 2005, Mayall was appointed an OBE in the Honours List.

In November 2008, Mayall announced on his website he was disbanding the Bluesbreakers, to cut back on his heavy workload and give himself freedom to work with other musicians. Three months later a solo world tour was announced, with: Rocky Athas on guitar, Greg Rzab on bass, and Jay Davenport on drums. Tom Canning, on organ, joined the band for the tour which started in March 2009. An album was released in September 2009. Since then, Mayall has continued to tour with the same backing band, minus Canning, who left due to other priorities.

Mayall has been married twice, and has six grandchildren. His second wife, Maggie Mayall, is an American blues performer, and since the early 1980s took part in the management of her husband’s career. The pair divorced in 2011 and Maggie wrote online about the experience.

In 2013, Mayall signed with producer Eric Corne’s label, Forty Below Records. The two have produced 4 studio albums together, A Special Life featuring accordionist C.J. Chenier, Find a Way to Care, Talk About That featuring Joe Walsh and Nobody Told Me. Corne also re-mastered some live recordings from 1967 featuring Peter Green, John McVie and Mick Fleetwood released as Live in 1967 Volumes I and II. In 2016, Mayall was inducted into the Blues Hall of Fame.

But Peter Green gave notice and soon started his own project, Peter Green’s Fleetwood Mac, which eventually was to include all three of Mayall’s Bluesbreakers at this time: Green, McVie, and drummer Mick Fleetwood who was a Bluesbreaker for only a few weeks. Two live albums, “Live in 1967” Volumes I and II, featuring this lineup were released on Forty Below Records in 2015 and 2016.

In 2018, Mayall made a new addition to his band; his first female lead guitarist, Carolyn Wonderland.

On June 25, 2019, The New York Times Magazine listed John Mayall among hundreds of artists whose material was reportedly destroyed in the 2008 Universal fire.

Mayall’s autobiography, Blues From Laurel Canyon: My Life As A Bluesman, co-written with author Joel McIver, was published by Omnibus Press in August 2019.