Lastimosa was one of the eight rookies of the Blue Eagles basketball team of the Ateneo de Manila University in the UAAP 1981-82 season, After a two-stint with Ateneo, Jojo transferred school when he enrolled at the University of San Jose Recoletos, a school in Cebu City. In June 1983, he joined elder brother Danny in the power-laden Mama's Love basketball club team. It was there where he got his biggest break and exposure, his reentry in the Manila basketball scene via the premier amateur league (PABL). In this league, he was able to showcase his skills in basketball. He leaves his audience astounded with his cat-quick reflexes, more so whenever he suspends himself in mid-air and a lot of Rizal Memorial Coliseum' habitués already likened him to the sensational Samboy Lim of Letran Knights.