After appearing in TV dramas and some minor roles in film, Joo was first cast as a lead in Dance Dance in 1999, for which he underwent extensive dance training. Although the film itself did not perform well, it gave Joo some publicity before he broke through with the box-office and critical hit Happy End. His role as a spurned lover in this psycho-drama attracted considerable notice in Korea, and the film itself also traveled to Hong Kong.

After some films he had been cast in were cancelled due to lack of financing, Joo did the 2003 boxing drama Punch with Shin Min-ah, then returned to the big screen in 2004, in the comedy Liar based on the play Run for Your Wife by Ray Cooney.

From 2004 to early 2005, Joo filmed the epic wuxia historical drama Bichunmoo (“Dance in the Sky”), but due to copyright issues with Korean broadcasters, it aired first in China and Taiwan in 2006. It was finally shown on Korean television in 2008, though SBS edited down the original 33 episodes into 14.

Meanwhile, Joo and Lee Yo-won’s 2005 TV series Fashion 70’s received good ratings of 30%. In 2006 he starred in Puzzle about a bank robbery gone wrong, and opposite Kim Ah-joong in the hugely popular romantic comedy 200 Pounds Beauty. The Kwak Kyung-taek gangster romance A Love co-starring Park Si-yeon followed in 2007.

He played a sports agent to a K-1 fighter in the 2009 TV series Dream, but it received low ratings for sharing the same timeslot as Queen Seondeok. The year after, Joo and Hallyu star Song Seung-heon appeared in A Better Tomorrow, the 2010 Korean remake of John Woo’s classic Hong Kong noir film. Joo was ranked fourth in CNNGo’s “South Korea’s Top 20 Hottest Male Celebs.”

Joo first sang “Like Rain, Like Music” by late singer Kim Hyun-sik during his first fan meeting in Japan at the Nakano Sun Plaza in Tokyo. He later released his cover of “Like Rain, Like Music” as a digital single in September 2011. Joo also starred in the accompanying music video with Go Joon-hee.

Joo and Ruby Lin starred in the 42-episode Chinese TV drama Flowers in Fog based on the novel by Qiong Yao (the title 花非花雾非雾 literally translates to “Flower is Not Flower, Fog is Not Fog”). It was shot in France, and aired on Hunan TV in 2013. He then returned to the Goryeo era to play a fictional character based on King Chunghye in Empress Ki, a historical drama with Ha Ji-won in the title role.

Jo made his theater debut in a 2015 staging of the musical Gone with the Wind, adapted from Margaret Mitchell’s novel. Joo said, “Rhett Butler is a character every actor would dream to play.” This was followed by melodrama series This is My Love with Kim Sa-rang (actress) on cable channel jTBC.

On November 2016, Joo signed with new management agency Huayi Brothers. He next played in the series Woman with a Suitcase, followed by Bad Guys 2 in 2017, and Big Issue in 2019.

Joo Jin-mo tied the knot with doctor Min Hye-yeon on June 1, 2019 in Jeju Island. Min is a Seoul National University graduate who specializes in family medicine. She has appeared on various health-related TV programs, in which she gained popularity and often dubbed as “Kim Tae-hee of the medical world.”

In January 2020, Joo Jin-mo revealed that he was a victim of phone hacking and was being blackmailed after several personal messages shared between Joo and his close actor friend appeared on an online community board on January 10, 2020. The messages allegedly included pictures of women and vulgar comments made by the two men about the women in the pictures. Joo’s agency has reported the case to authorities. Investigation is ongoing. He also apologized to the women mentioned in the messages.