Pulver was born in Southend-on-Sea, Essex. Her father is from a Jewish family and her mother converted to Judaism. Her parents separated when she was eleven. Pulver attended the National Youth Music Theatre between 1994 and 1998, as well as The Liz Burville Dance Centre, Bexley. In 1997, she began studying at the Doreen Bird College of Performing Arts, and graduated in 2000. She has since worked as an actress, singer and dancer in West End musicals.

In 2003, Pulver met American actor Josh Dallas while he was in the UK studying at the Mountview Academy of Theatre Arts. They married over Christmas 2007 in a 16th-century barn in Devon. The couple honeymooned in the Maldives. Dallas confirmed their divorce in an interview on Bob Rivers’s radio show on 2 December 2011.

In 2008, Pulver recorded a song for the CD Act One – Songs from the Musicals of Alexander S. Bermange. Pulver joined the cast of the BBC’s Robin Hood in 2009 as Isabella, the sister of Guy of Gisbourne.

In 2010, Pulver joined the cast of the third series of HBO’s True Blood as Sookie Stackhouse’s fairy godmother, Claudine Crane.

In 2012 she joined the cast of Da Vinci’s Demons as the “seductive and politically-minded” Clarice Orsini, a series regular and wife of Lorenzo Medici.

Pulver began dating actor Raza Jaffrey in 2012 and the two were married on 27 December 2014. She currently resides in Los Angeles.

In 2014, she played Louise in a revival of Gypsy at the Chichester Festival Theatre. The production transferred to the West End in April 2015. Miss Pulver won the 2016 Olivier Award for Best Supporting Actress in a Musical for the same role in the Savoy Theatre production of Gypsy. Louise is actually the title role, as “Louise” becomes the real-life early-20th-century American strip-tease sensation Gypsy Rose Lee, on whose memoirs the musical is based. Pulver’s co-star in the Savoy production, Imelda Staunton, won the 2016 Olivier Award for Best Actress in a Musical for her role as the larger-than-life Mama Rose.

Pulver played Erin Watts, the new chief of Section D, in the tenth and final series of BBC spy drama Spooks, a role she reprised in the 2015 feature film Spooks: The Greater Good

The couple had a son named Ozias in 2017. On her 40th birthday, Lara announced via Twitter that she was pregnant with the couple’s second child.