In 1508 Ariosto’s play Cassaria appeared, and the next year I suppositi [it] was first acted in Ferrara and ten years later in the Vatican. A prose edition was published in Rome in 1524, and the first verse edition was published at Venice in 1551. The play was translated by George Gascoigne and acted at Gray’s Inn in London in 1566 and published in 1573, which was later used by Shakespeare as a source for The Taming of the Shrew.

Lodovico Ariosto is featured in the novelization of Assassin’s Creed: Revelations (the novel describes Ezio’s journey to Masyaf, his marriage to Sofia Sartor, the birth of his children and his retirement in more detail) as an Assassin. When Ezio retires after the events of the game, in 1513, he gives his position of Mentor to Lodovico.

In 1516 the first version of the Orlando Furioso in 40 cantos, was published at Ferrara. The third and final version of the Orlando Furioso, in 46 cantos, appeared on 8 September 1532.

The cardinal went to Hungary in 1518, and wished Ariosto to accompany him. The poet excused himself, pleading ill health, his love of study, and the need to care for his elderly mother. His excuses were not well-received, and he was denied even an interview. Ariosto and d’Este got into a heated argument, and Ariosto was promptly dismissed from service.