María Isabel was born in Ayamonte, Province of Huelva, Spain. She demonstrated an interest in dancing and singing from a very young age. Her love for singing and dancing took her to the 2004 Spanish Eurojunior competition, which was televised nationally in her native Spain. The purpose of this competition was to select Spain’s representative for the relatively new Junior Eurovision Song Contest competition, which began in 2003.

“Antes Muerta que Sencilla” became an instant radio hit in Spain, and was later promoted in Latin America, where María Isabel’s album was released in 2005. Milly Cangiano, a Puerto Rican television show host, made the song’s title one of the catchphrases she uses daily during her show.

In addition, María Isabel began her first tour of Latin America and the United States after the album’s release. Her song became a chart-topper in Argentina, Colombia, and Puerto Rico, among others. In April 2005, she was interviewed on US nationwide television for the first time, when she was presented on Univision’s El Gordo y la Flaca and chatted with hosts Lili Estefan and Raúl De Molina.

Her second album was titled Número 2. It includes the songs “Pues va a ser que no” and “En mi jardín”. These songs are part of the Spanish soundtrack of the Barbie film Barbie and the Magic of Pegasus. Her third album, Capricornio, was released on 21 November 2006 and peaked at number 11 on the Spanish Albums Chart. It is a double disc set which also includes a DVD with all of her videoclips and extras.

In 2009 and 2010 she hosted the children’s show Los Lunnis on Televisión Española. In November 2009 she released her fifth album, Los Lunnis con María Isabel, which contained the songs from the show as well as two original tracks.

After a career hiatus in order to finish her studies, María Isabel returned to music in 2015, eleven years after her debut, with her sixth studio album, Yo Decido.

On 29 December 2015, María Isabel was announced as one of the six candidates to represent Spain in the Eurovision Song Contest 2016. She took part in the televised national final organised by Televisión Española with a song composed by David Santisteban and titled “La vida sólo es una”, the first single from Yo Decido. She came 4th with 68 points.

On 5 July 2019, María Isabel released a single titled “Tu Mirada”.