Rileah Vanderbilt is largely given credit to bringing the four women of Team Unicorn together. She and Clare Grant produced geek videos together and won two awards at the 2009 Star Wars Fan Film Awards for their short “Saber”. Vanderbilt became friends with Milynn Sarley in 2009, and Michele Boyd was introduced in January 2010.

The group released its debut parody song “G33k & G4m3r Girls” online in September 2010. The music video, spoofing Katy Perry’s “California Gurls”, is filled with geek cultural references and pays tribute to women who love gaming, manga, and science fiction. The video reached 1 million views in its first week online, but met with some mixed responses to its portrayal of the women.

In 2013, Adult Swim announced a development agreement with the creators of Robot Chicken for a Team Unicorn live action/animation hybrid pilot called The Team Unicorn Saturday Action Fun Hour! which introduces Alison Haislip as the new Blue Unicorn, and stars Jane Lynch, Alan Tudyk, Tara Strong, and Kevin Shinick.

On December 7, 2014, Team Unicorn released “All About That Base”, a parody of the Meghan Trainor song “All About That Bass”, with the tagline lyric being “No Rebels”. The video has a Star Wars theme and featured stormtroopers dancing alongside cheerleaders dressed in attire inspired by Darth Vader. Mitchell Peters of Billboard opined that the parody was “catchy” and “hilarious”, while Donna Dickens of HitFix questioned whether the video sent the wrong message by “tacitly saying it’s okay for geek girls to exist, only so long as they are acceptably ‘hot'”.