She played in her first LPGA Tour event in 1997 as an amateur at the age of 14 (handicap of 2). Gulbis was the top player on the boys’ golf team at Granite Bay High School and graduated at age 16. She then accepted a golf scholarship to the University of Arizona, the 2000 national champions, where she was a teammate of fellow freshman, Lorena Ochoa. After one season at Arizona, Gulbis left college to turn professional in July 2001 at age 18. At the LPGA Final Qualifying Tournament in October 2001 at Daytona Beach, Florida, Gulbis finished tied for third to earn her card for the 2002 season.

Although Gulbis did not win a tournament until her sixth season on tour, she finished sixth on the LPGA money list in her fourth season with over $1 million in earnings in 2005. She placed in the top 10 in four consecutive major championships from the 2005 LPGA Championship to the 2006 Kraft Nabisco Championship.

In 2006, Gulbis began writing a monthly advice column in FHM. In November 2005 a reality television show, The Natalie Gulbis Show, made its debut on The Golf Channel. The show had its second-season premiere on October 18, 2006. Gulbis also has appeared on the 2007 version of Tiger Woods PGA Tour by EA Sports, along with fellow professionals, Annika Sörenstam, Ian Poulter, and Luke Donald, among others. In August 2007 Gulbis appeared on the August/September cover of Sactown Magazine in an article that profiled the rising star’s busy life of product endorsements and photo shoots, though, no pro win. Gil Ozir, Vice President of Marketing for Raymond Weil, a luxury watchmaker and one of Gulbis’ endorsement deals, was quoted as saying, “Once she starts winning, she’s going to be a megastar”. A few days later, Gulbis won the Evian Masters, her first professional LPGA title.

In 2009, Gulbis appeared in the second season of Celebrity Apprentice. Throughout the season, each celebrity raised money for a charity of their choice; Gulbis selected the Boys and Girls Club. She was fired on the April 19, 2009 episode of the show.

In 2010, she appeared in the tenth season of CSI: Crime Scene Investigation at the 12th episode “Long Ball”.

Gulbis appeared in the 2012 Sports Illustrated Swimsuit Issue wearing only body paint.

In July 2013, Gulbis became engaged to Josh Rodarmel, a former quarterback for Yale University. They were married in December.

^ The Evian Championship was added as a major in 2013

In 2016, Gulbis spoke at the Republican National Convention in support of Donald Trump. There was speculation that she would run for the open 3rd congressional district of Nevada as a Republican in 2018.

In May 2018, President Donald Trump appointed Gulbis to serve as a member of his Council on Sports, Fitness & Nutrition.