Since 1985, Greene and her husband, Mike Smith were directors of Flying TV Ltd, an aviation video company operating helicopters based at Denham Aerodrome, Denham, Buckinghamshire. Since the death of Mike Smith, Greene remains the Director of the company.

On 10 September 1988, Greene and her partner Mike Smith, the television and radio presenter, were seriously injured in a helicopter accident in Gloucestershire. The couple spent a number of weeks in hospital before Greene returned to present Going Live! while still in plaster.

In addition to presenting magazine shows such as the BBC’s Pebble Mill and Good Morning Summer alongside Will Hanrahan, Greene acted in the Doctor Who serial Attack of the Cybermen, Casualty, Brookside and French & Saunders. In 1992 she combined her talents in a hoax paranormal programme called Ghostwatch, alongside Smith and talk-show host Michael Parkinson. Greene and Smith made a foray into feature films in The Man Who Knew Too Little (1997), starring Bill Murray; they played presenters of The Theatre Of Life, a fictional British TV show which promised the participant a role in a crime drama.

In 1996, Greene began a four-year partnership with the Food Network, hosting a daily news, debate and magazine show. Later, Greene appeared in the ITV programme Have I Been Here Before? and was also a regular presenter on magazine show This Morning. She also made a guest appearance in the final episode of The Harry Hill Show. She has also presented Discovery Home & Health’s Weddings Live, an annual 6-hour broadcast which showcases several weddings live from across the country, from 2005 to 2007. Fellow presenters include Brendan Courtney and celebrity makeup artist and stylist Armand Beasley. Partnered with Fred Palascak, Greene competed in the 2008 ITV Dancing on Ice competition.

Greene took part in an episode of BBC Wales’s Coming Home programme, first shown on 22 December 2010. Her mother had died a month before filming but she decided to continue the process, alongside her 87-year-old father. The programme was able to trace her family tree back to the 17th century in Wales.

Greene and her husband, Mike Smith, appeared on Pointless on BBC One in December 2012. Smith died on 1 August 2014, following complications after major heart surgery.

Greene became ambassador for ovarian cancer charity Target Ovarian Cancer in 2012.

In April 2017, Greene guest presented an episode of This Morning alongside Phillip Schofield. She returned to guest present the show in July 2017, and again on 4 August 2017 and on 18 August 2017, alongside Rylan Clark-Neal.