Usha was born into Tamil family in Mumbai in 1947. Her father was Vaidyanath Someshwar Sami who hailed from Chennai, in Tamil Nadu.

In 1968, she recorded covers of two pop songs in English, “Jambalaya” and The Kingston Trio’s “Greenback Dollar”, on an EP, Love Story, and “Scotch and Soda”, another Kingston Trio song, which sold very well in the Indian market. She also spent some time in London during this early period. She was a frequent visitor to Vernon Corea’s BBC office at the Langham in London and was interviewed on London Sounds Eastern on BBC Radio London. Usha visited Nairobi as part of an Indian festival. She was so popular that she was invited to stay on. Singing and quite often nationalistic songs in Swahili made her extremely popular and the then President Jomo Kenyatta made her an Honorary Citizen of Kenya. She sang the famous song “Malaika” (‘Angel’) with Fadhili Williams who was the original singer. She produced a record “Live in Nairobi” with a local band Fellini Five.

Usha Uthup started her music career in Chennai in 1969, singing in a small nightclub called Nine Gems in the basement of the erstwhile Safire theatre complex on Mount Road, wearing a saree and leg callipers. Her performance was so well received that the owner of the nightclub asked her to stay on for a week. After her first night club gig, she began singing in Calcutta at night clubs such as “Trincas”. She met her future husband Uthup in Trincas. At about the same time, she also sang at “Talk of the Town”, now known as “Not Just Jazz by the Bay” in Bombay (now Mumbai). After Trincas, her next engagement took her to Delhi where she sang at the Oberoi hotels. By happenstance, a film crew belonging to Navketan unit and Dev Anand visited the nightclub and they offered her a chance to sing movie playback. As a result, she started her Bollywood career with Ivory-Merchant’s Bombay Talkies (1970) in which she sang an English number under Shanker-Jaikishan and then Hare Rama Hare Krishna. Originally, she was supposed to sing Dum Maro Dum along with Asha Bhosle for Hare Rama Hare Krishna. However, as a result of internal politicking on the part of other singers, she lost that chance but ended up singing an English verse.

In addition, she has also sung playback for the Bollywood movies Revolver Rani (2014), Dhol (2007), June R (2005), Joggers’ Park (2003), Jajantaram Mamantaram (2003), Ek Tha Raja (1996), Dushman Devta (1991), Bhavani Junction (1985), Hum Paanch (1980), and Purab Aur Paschim (1970) among others. She has also sung two English songs “We have got” and “How can I tell you” in the 1977 Kannada film Sangharsha.

Uthup is also an actress. In 2006, she acted in the Malayalam movie Pothan Vava as Kurisuveettil Mariamma.

She recorded her first album with Luis Banks for which she was paid Rs. 3500. Since then, she has recorded numerous albums. Usha’s Hindi version of Michael Jackson’s “Don’t Stop Til You Get Enough”, titled “Chhupke Kaon Aya”, can be found on the album Tom Middleton – The Trip (2004). A cover of Gloria Gaynor’s “I Will Survive” is on another Tom Middleton album, Cosmosonica – Tom Middleton Presents Crazy Covers Vol. 1 (2005). She recorded a song called “Rhythm and Blues” with the Indian rock band Parikrama which appeared on Channel V on 23 April 2007.

She made a cameo appearance in the movie Bombay To Goa. In 2007, she appeared in Bow Barracks Forever directed by Anjun Dutt as herself. Again in 2007, she appeared in Hattrick music video as herself.

She has a minor role in the 2010 Tamil movie Manmadan Ambu.

She also acted in Vishal Bhardwaj’s 7 Khoon Maaf as a maid. She has also sung a song in the film which released on 18 February 2011. In 2012, she has starred in a Kannada film Parie.

She is a stage performer and gave performances all over the world and is known for her lively stage presence. She has been felicitated with several awards over the years, some of which include Rajiv Gandhi Purashkar for National Integration for quality music, Mahila Shiromani Purashkar for international peace, and Channel [V] award for outstanding achievement. She appeared on the Kapil Sharma Show on 26 May 2019

In 2019, she appeared in the documentary If Not for You for which, she recorded a cover of “Blowin’ in the Wind” by legendary singer-songwriter Bob Dylan.